195 Follicular wave synchronisation with oestradiol Benzoate or gonadotrophin-releasing hormone for ovum pickup in lactating Holstein cows

Abstract:

Several strategies have been tested to improve in vitro embryo production efficiency in cattle. A successful strategy has been the synchronisation of the follicular wave with protocols based on progesterone and oestradiol benzoate (EB); however, EB is banned in several countries and will soon be banned in others. This study aimed to compare the use of gonadotrophin-releasing hormone (GnRH) and EB in the synchronisation of the follicular wave before OPU. A total of 12 lactating Holstein cows aged 3–8 years were divided into two treatments in a 2× 2 crossover design in four replicates. On Day 0, cows received a P4 implant of 0.6 g (Disprogest, Zoovet®) and 150 µg of D-cloprostenol (Dextrogenol, Von Franken®), then were randomly divided into two groups; the BE group received 2 mg of Oestradiol benzoate (Von Franken®) and the GnRH group received 10.5 µg of Buserelina acetate (Gestar, Over®). On Day 5 …

Año de publicación:

2022

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Fuente:

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