Evaluation of nCoV-QS (MiCo BioMed) for RT-qPCR detection of SARS-CoV-2 from nasopharyngeal samples using CDC FDA EUA qPCR kit as a gold standard: An example of the need of validation studies
Abstract:
Background: Several qPCR kits are available for SARS-CoV-2 diagnosis, mostly lacking of evaluation due to covid19 emergency. Objective: We evaluated nCoV-QS (MiCo BioMed) kit using CDC kit as gold standard. Results: We found limitations for nCoV-QS: 1) lower sensitivity 2) lack of RNA quality control probe. Conclusions: Validation studies should be implemented for any SARS-CoV-2 RT-qPCR commercial kit to prevent unreliable diagnosis.
Año de publicación:
2020
Keywords:
- Validation
- CdC
- Rt-qPCR
- SARS-COV-2
Fuente:


Tipo de documento:
Article
Estado:
Acceso restringido
Áreas de conocimiento:
- Microbiología
- Microbiología
Áreas temáticas:
- Enfermedades
- Medicina y salud