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Evaluation of neuronal cell death by laser scanning cytometry

Abstract:

We developed a method in which laser scanning cytometry (LSC) is applied to evaluate cell viability. Neuronal cell death induced by glutamic acid, serum potassium deprivation and 3-nitropropionic acid was studied in cerebellar granule cells by neutral red assay (NR) and LSC, using propidium iodide (PI) as fluorescent dye. PI labeled the nuclei of dead neurons and increased fluorescence was measured using a laser scanning cytometer. Similar levels of damage for each injury were detected by NR or LSC. The protocol presented here, provides a fast and sensitive assay for the analysis of neuronal viability using LSC, and can be used to study new neuroprotective drugs in neuronal cell cultures. © 2002 Elsevier Science B.V. All rights reserved.

Año de publicación:

2002

Keywords:

    Fuente:

    scopusscopus

    Tipo de documento:

    Article

    Estado:

    Acceso restringido

    Áreas de conocimiento:

    • Biología celular
    • Neurología

    Áreas temáticas:

    • Enfermedades
    • Sistemas fisiológicos específicos de los animales
    • Fisiología humana

    Contribuidores:

    Pallás M.Pallás M.
    Pubill D.Pubill D.
    Verdaguer E.Verdaguer E.
    Antoni CaminsAntoni Camins
    Rimbau V.Rimbau V.
    Camarasa J.Camarasa J.
    Escubedo E.Escubedo E.
    Jiménez A.Jiménez A.