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Experimental transmission of the mild strain of Tomato yellow leaf curl virus (TYLCV) to Amaranthus dubius by Bemisia tabaci

Abstract:

Tomato yellow leaf curl virus (TYLCV) is a monopartite begomovirus from the Old World. The mild strain of this virus (TYLCV-Mld) was described for South America in Venezuela in 2007. Due to the potential risks of establishment of this virus in the field, six common weeds were evaluated for susceptibility to an isolate of TYLCV-Mld by using adults of Bemisia tabaci (Hemiptera: Aleyrodidae) to mediate viral transmission. In this work, detection based on PCR amplification with TYLCV specific primers showed Amaranthus dubius (Amaranthaceae) as the only infected weed. In A. dubius, viral symptoms were observed from 11. 0 ± 1. 3 days post-inoculation and the transmission rate of TYLCV-Mld to this plant species was 83. 3%. The successful back-transmission of TYLCV-Mld from A. dubius to tomato plants was demonstrated. © 2012 Springer Science + Business Media B.V.