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Exploring AOX gene diversity

Abstract:

In angiosperms alternative oxidase (AOX) has been described as a small multigene family composed of three to five genes distributed in two different subfamilies, AOX1 and AOX2. Nevertheless, while AOX1 occurs in both monocots and eudicots, AOX2 occurs only in eudicots. Neimanis et al. recently suggested that the lack of AOX2 in monocots is due to a secondary gene loss event during evolution. AOX has recently been proposed as a key enzyme coordinating phenotypic changes related to adaptation to environmental changes (plant plasticity), and was for that reason considered as a target for functional marker (FM) development. An analysis pipeline for FM discovery using artificial intelligence (AI) was developed. Training data consisted of AOX data from both clone (phased) and amplicon (unphased) Sanger sequencing data. AOX is a terminal quinol oxidase found in the mitochondrial electron transport chain (ETC) that introduces a branch-point at the level of ubiquinol.