Fas ligand+ fallopian tube epithelium induces apoptosis in both Fas receptor+ T lymphocytes and endometrial cells

Abstract:

Objective To establish whether human fallopian tube (FT) epithelium can induce apoptosis in T lymphocytes and endometrial cells. Design Laboratory-based study. Setting Hospital. Patient(s) Women undergoing abdominal hysterectomy for FT samples, and women volunteers with and without endometriosis for endometrial biopsies. Intervention(s) FT samples obtained at time of surgery performed in reproductive-aged women with normal menstrual cycles. Main Outcome Measure(s) T lymphocytes or endometrial cells coincubated with FT epithelial cells and assayed for apoptosis by DNA nick-end labeling and caspase-3 activity, with the presence of Fas ligand (FasL) and Fas receptor (FasR) assessed by indirect immunostaining. Result(s) The epithelium of the FT-induced apoptosis in T cells as well as in human endometrial cells. The mechanism probably involves the FasL/FasR system; accordingly, we observed FasL at …

Año de publicación:

2013

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Fuente:

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