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Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples

Abstract:

In low-resource settings, resilience to infectious disease outbreaks can be hindered by limited access to diagnostic tests. Here we report the results of double-blinded studies of the performance of paper-based diagnostic tests for the Zika and chikungunya viruses in a field setting in Latin America. The tests involved a cell-free expression system relying on isothermal amplification and toehold-switch reactions, a purpose-built portable reader and onboard software for computer vision-enabled image analysis. In patients suspected of infection, the accuracies and sensitivities of the tests for the Zika and chikungunya viruses were, respectively, 98.5% (95% confidence interval, 96.2–99.6%, 268 serum samples) and 98.5% (95% confidence interval, 91.7–100%, 65 serum samples) and approximately 2 aM and 5 fM (both concentrations are within clinically relevant ranges). The analytical specificities and sensitivities of the tests for cultured samples of the viruses were equivalent to those of the real-time quantitative PCR. Cell-free synthetic biology tools and companion hardware can provide de-centralized, high-capacity and low-cost diagnostics for use in low-resource settings.

Año de publicación:

2022

Keywords:

    Fuente:

    scopusscopus

    Tipo de documento:

    Article

    Estado:

    Acceso abierto

    Áreas de conocimiento:

    • Microbiología
    • Microbiología

    Áreas temáticas:

    • Enfermedades
    • Microorganismos, hongos y algas

    Contribuidores:

    Castellanos J.E.Castellanos J.E.
    Homme P.Homme P.
    Krokovsky L.Krokovsky L.
    Sutyrina P.Sutyrina P.
    Green A.A.Green A.A.
    Karlikow M.Karlikow M.
    Calderón-Peláez M.A.Calderón-Peláez M.A.
    Paiva M.Paiva M.
    Souza B.N.R.F.Souza B.N.R.F.
    Pena L.Pena L.
    Rackus D.G.Rackus D.G.
    da Silva S.J.R.da Silva S.J.R.
    Ma D.Ma D.
    Victoria E. NipázVictoria E. Nipáz
    Patricio PoncePatricio Ponce
    de Magalhães J.J.F.de Magalhães J.J.F.
    Benitez A.D.Benitez A.D.
    Cevallos V.E.Cevallos V.E.
    Jaenes K.Jaenes K.
    Pardee K.Pardee K.
    Camacho-Ortega S.Camacho-Ortega S.
    de Albuquerque Cabral D.G.de Albuquerque Cabral D.G.
    Ayres C.Ayres C.
    Cicek S.Cicek S.
    Guo Y.Guo Y.
    Collins J.J.Collins J.J.
    Xu T.Xu T.
    Ferrante T.Ferrante T.
    Xiong Y.Xiong Y.