First report of Shallot virus X in garlic in Ecuador

Abstract:

Garlic (Allium sativum L.) is widely cultivated in the Andean region of Ecuador. Viral diseases of garlic are widespread and losses in crop yields due to virus infection are a serious problem because they are propagated vegetatively (Kamenetsky and Rabinowitch 2001). Shallot virus X (ShVX) is a known pathogen of species belonging to the genus Allium. ShVX was first detected in shallot in Russia (Kanyuka et al. 1992). Thirty garlic leaf samples from both symptomless (10) and symptomatic (20) plants (showing chlorotic streaking and leaf curling) were collected during July 2015 from Alausí (Chimborazo Province) across multiple fields. Seventeen out of 30 garlic samples tested by double antibody sandwich ELISA using antibodies for ShVX (DSMZ AS-1042) showed a positive reaction and 13 of the ELISA-positive samples showed symptoms of the disease. Total RNA was extracted from 100 mg of 10 infected plant …

Año de publicación:

2017

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Fuente:

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