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First report of tomato spotted wilt virus infecting Chrysanthemum in Ecuador

Abstract:

The natural occurrence of Tomato spotted wilt virus infecting chrysanthemum was initially detected by triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) and further confirmed by reverse transcription polymerase chain reaction (RT-PCR) using tospovirus universal primers. The sequence analysis of the PCR products showed 95.6 % and 95.0 of maximum identity at nucleotide and amino acid levels respectively with TSWV chrysanthemum isolate from Ecuador (KT590402). This is the first report of the natural occurrence of TSWV on chrysanthemum in Ecuador.