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Flow perfusion improves seeding efficiency in scaffolds for bone tissue engineering

Abstract:

Bone grafts have been generated under a wide variety of culturing conditions, including static and dynamic systems by seeding and culturing osteoblastic cells on porous scaffolds. Scaffold seeding with osteoblastic cells affects the development of bone grafts, determining initial cell number and cell spatial distribution in the scaffold. Spinner flasks and rotating wall vessels have been used as dynamic systems, but improvement is desirable. We evaluate the effect of perfusion on seeding efficiency and spatial distribution of osteoblastic cells in fibrous polymeric matrices and foams. The effect of fiber size and surface modification by oxygen plasma treatment is also evaluated. MC3T3-E1 cells were seeded statically and in a flow perfusion bioreactor. Dynamic seeding resulted in the most efficient seeding technique. The total number of cells attached to the scaffold decreased as the fiber size increased, but a direct relation was observed between fiber size and cell surface density. © 2005 Taylor & Francis Group, London.