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Fostering the action of versatile peroxidase as a highly efficient biocatalyst for the removal of endocrine disrupting compounds

Abstract:

Response surface methodology (RSM) was used to optimize the removal of five endocrine disrupting compounds (EDCs) by the enzyme versatile peroxidase (VP): bisphenol A (BPA), triclosan (TCS), estrone (E1), 17β-estradiol (E2) and 17α-ethinylestradiol (EE2). The optimal variables of enzyme activity (90-100UL-1), sodium malonate (29-43mM) and MnSO4 (0.8-1mM) led to very high removal rates of the five pollutants (2.5-5.0mgL-1min-1). The structural elucidation of transformation products arising from the enzymatic catalysis of the EDCs was investigated by Gas Chromatography coupled to Mass Spectrometry (GC-MS) and Liquid Chromatography Electrospray Time-of-Flight Mass Spectrometry (LC-ESI-TOF-MS). The presence of dimers and trimers, indicative of oxidative coupling, was demonstrated.