Fostering the action of versatile peroxidase as a highly efficient biocatalyst for the removal of endocrine disrupting compounds
Abstract:
Response surface methodology (RSM) was used to optimize the removal of five endocrine disrupting compounds (EDCs) by the enzyme versatile peroxidase (VP): bisphenol A (BPA), triclosan (TCS), estrone (E1), 17β-estradiol (E2) and 17α-ethinylestradiol (EE2). The optimal variables of enzyme activity (90-100UL<sup>-1</sup>), sodium malonate (29-43mM) and MnSO<inf>4</inf> (0.8-1mM) led to very high removal rates of the five pollutants (2.5-5.0mgL<sup>-1</sup>min<sup>-1</sup>). The structural elucidation of transformation products arising from the enzymatic catalysis of the EDCs was investigated by Gas Chromatography coupled to Mass Spectrometry (GC-MS) and Liquid Chromatography Electrospray Time-of-Flight Mass Spectrometry (LC-ESI-TOF-MS). The presence of dimers and trimers, indicative of oxidative coupling, was demonstrated.
Año de publicación:
2016
Keywords:
Fuente:
scopusTipo de documento:
Article
Estado:
Acceso restringido
Áreas de conocimiento:
- Bioquímica
- Bioquímica
- Biotecnología
Áreas temáticas de Dewey:
- Microorganismos, hongos y algas
- Ecología
- Ingeniería química
Procesado con IAObjetivos de Desarrollo Sostenible:
- ODS 6: Agua limpia y saneamiento
- ODS 12: Producción y consumo responsables
- ODS 3: Salud y bienestar
Procesado con IA