Functional Characterization of a Cysteine‐Less Isoform of the Human GLUT2 Hexose Transporter
Abstract:
The GLUT2 carrier is the major glucose transporter isoform expressed in hepatocytes, insulin‐secreting pancreatic beta cells, and absorptive epithelial cells of the intestinal mucosa and kidney. GLUT2 is suggested to play a critical role in maintaining glucose homeostasis because it functions as a low affinity, high‐turnover transport machinery. However, little is known regarding the mechanism of transport at the structural level. Here, we designed a cysteine‐less isoform of GLUT2 (C‐less GLUT2) to serve as platform for functional and structural studies, i.e. cysteine‐scanning mutagenesis in combination with thiol chemical modification. The kinetic properties of the C‐less GLUT2 expressed into Xenopus oocytes were like those of the native GLUT2, except that the overall activity was lower than that of the wild‐type transporter. Like the native protein, the C‐less GLUT2 transported methylglucose and deoxyglucose …
Año de publicación:
2017
Keywords:
Fuente:
Tipo de documento:
Other
Estado:
Acceso abierto
Áreas de conocimiento:
- Bioquímica
- Bioquímica
- Bioquímica
Áreas temáticas:
- Fisiología humana
- Fisiología y materias afines
- Mammalia