GENE THERAPIES AND VACCINES AGAINST INFECTIOUS DISEASES

Abstract:

Background: several lines of evidence suggest a potential role for IFN alpha (IFN-α) in control of HIV-1 replication. However, recombinant IFN-α protein is only moderately effective in inhibiting HIV, and such inhibition is reversible when the IFN therapy is withdrawn. We hypothesized that stable expression of human IFN-α2, delivered by recombinant SV40 vectors (rSV40), and conditional on HIV infection, could provide sustained protection from HIV. Methods: to achieve high levels of expression of IFN-α2 at the site of infection, we devised a recombinant, Tag-deleted, SV40-derived gene transfer vector, SV [HIVLTR] IFN, in which expression of human IFN-α2 would be driven by the HIV-1 LTR and so be trans-activated by HIV infection. Human T lymphocyte cell lines and primary human blood lymphocytes (PBL) were transduced with SV [HIVLTR] IFN. No selection was used. There was no detectable toxicity. These and …

Año de publicación:

2002

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