Generation of human elongating multi-lineage organized cardiac gastruloids
Abstract:
Human elongating multi-lineage organized (EMLOC) gastruloid technology captures key aspects of trunk neurodevelopment including neural integration with cardiogenesis. We generate multi-chambered, contractile EMLOC gastruloids with integrated central and peripheral neurons using defined culture conditions and signaling factors. hiPSC colonies are primed by activating FGF and Wnt signaling pathways for co-induced lineages. EMLOC gastruloids are then initialized with primed cells in suspension culture using timed exposure to FGF2, HGF, IGF1, and Y-27632. Cardiogenesis is stimulated by FGF2, VEGF, and ascorbic acid. For complete details on the use and execution of this protocol, please refer to Olmsted and Paluh (2022).1
Año de publicación:
2022
Keywords:
- Neuroscience
- Developmental biology
- stem cells
- Organoids
- bioinformatics
Fuente:
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Tipo de documento:
Article
Estado:
Acceso abierto
Áreas de conocimiento:
- Biología del desarrollo
Áreas temáticas:
- Fisiología humana
- Fisiología y materias afines
- Anatomía humana, citología, histología