Generation of human elongating multi-lineage organized cardiac gastruloids


Abstract:

Human elongating multi-lineage organized (EMLOC) gastruloid technology captures key aspects of trunk neurodevelopment including neural integration with cardiogenesis. We generate multi-chambered, contractile EMLOC gastruloids with integrated central and peripheral neurons using defined culture conditions and signaling factors. hiPSC colonies are primed by activating FGF and Wnt signaling pathways for co-induced lineages. EMLOC gastruloids are then initialized with primed cells in suspension culture using timed exposure to FGF2, HGF, IGF1, and Y-27632. Cardiogenesis is stimulated by FGF2, VEGF, and ascorbic acid. For complete details on the use and execution of this protocol, please refer to Olmsted and Paluh (2022).1

Año de publicación:

2022

Keywords:

  • Neuroscience
  • Developmental biology
  • stem cells
  • Organoids
  • bioinformatics

Fuente:

scopusscopus

Tipo de documento:

Article

Estado:

Acceso abierto

Áreas de conocimiento:

  • Biología del desarrollo

Áreas temáticas:

  • Fisiología humana
  • Fisiología y materias afines
  • Anatomía humana, citología, histología