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Genetic diversity of the Venezuelan caricaceae family collection

Abstract:

The genetic diversity of Venezuelan Caricaceae family collection was studied using 15 random primers of the series OPA, OPK, OPM, OPY, OPO and OPW from Operon Technologies Inc, and the amplification fragments linked with the discrimination among Carica papaya L. and three species of Vasconcellea genera: V. cundinamarcensis Linden, V. cauliflora Jacq and V. microcarpa Jacq (subspecies microcarpa and pilífera) were identified. The 15 RAPDs markers produced 403 polymorphic amplification fragments with sizes between 186-2550 base pairs (bp), a reproducibility >90%, and allowed a clear separation of the four species, using the simple matching coefficient and the groups obtained from the UPGMA cluster algorithm applied to the first three principal axes. After external logistic biplot fitting, nine amplification fragments were selected: OPA- 07(466pb), OPY-07(640pb), OPY-09(390pb), OPO-10(680pb), OPW-06(230pb) and OPW-06(420pb), directly involved in the classification of the two genera; and OPA-02(1111pb), OPA- 07(675pb) and OPW-06(740pb) allowing to distinguish among Vasconcelleas species. The analysis did not detect any pattern associated with the geographic origin of the entrances. The results obtained for Venezuelan Caricaceae collection using RAPDs markers are similar to those reported by several authors in morphological, biochemistry and molecular studies, and in intergeneric crosses, and generated information to facilitate the handling of the collection, choosing of parentals and selection in breeding programs.