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Genetic fingerprint of wild and commercial genotypes of Passiflora spp. by using RAPD patterns

Abstract:

An important collection of cultivated and wild species of Passiflora is maintained at the Germplasm Bank of INIA-CENIAP in Maracay, Venezuela, and it is essential to know the genetic identity of such materials in order to avoid duplicates. Sixty primers from the Operon series were used to determine the genetic fingerprint of 17 genotypes and identify patterns of bands for each genotype. Displayed bands by each genotype were numbered in ascending and sequential order thus creating a distinctive genetic pattern for each genotype in relation to each primer used, as well as the primer characterization in relation to each genotype studied. The genetic analysis established that RAPDs allowed generating a set of specific band patterns characteristic for each genotype that becomes their genetic fingerprint. Nineteen primers with a high discriminatory potential were chosen and should be the base of further genetic studies of Passiflora genetic diversity. These primers permitted observing different positions of similar bands among the individuals suggesting the existence of several alleles for these characteristics. RAPD patterns allowed separating cultivated materials from wild types, finding a high degree of variability among the cultivated materials.