Genetic transformation of potato cultivars using R genes to increase resistance to late blight of potato caused by Phytophthora infestans [Conference poster].

Abstract:

Several resistance (R) genes have recently been isolated and used in a transgenic approach. The RB gene of Solanum bulbocastanum confers partial resistance to many isolates of Phytophthora infestans, the causal agent of late blight (LB) disease in potato. We have selected three recipient varieties:(1) Desiree, which is easy to transform and has wide adaptability;(2) Granola which is the principal fresh market variety in Indonesia; and (3) Victoria (Asante) which is a well adopted variety in Uganda (and Kenya). Genetic transformation of Desiree was done with 2 batches of events. The first one resulted in 14 putatively transformed plants regenerated from wounded leaves and internodes. Out of these, 10 transgenic events have been selected as being independent events and 7 of them devoid of non-TDNA backbone vector sequences. These will be field trialed to determine their level of resistance to LB and other agronomical characteristics. The second batch resulted in 41 putative transformed plants. These plants are currently undergoing molecular characterization. With the variety Granola, 15 putative transformed events with the RB gene construct were produced. This variety displays much lower regeneration efficiency in addition to rapid oxidation of explants. Finally, the variety Victoria is being tested for regeneration efficiency and susceptibility to kanamycin and carbenicillin to select for transformed events while eliminating Agrobacterium cells. Two additional R genes, Rpi-blb2 and Rpi-vnt1. 1 from S. bulbocastanum and S. venturii respectively, are now used in combination with RB gene in order to increase durability of resistance to late blight.

Año de publicación:

2009

Keywords:

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