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Genome-Wide Identification and Comprehensive Analyses of TCP Gene Family in Banana (Musa L.)

Abstract:

Teosinte Branched, Cycloidea, Proliferating Cell Factor proteins are a large family of transcription factors in angiosperms and are specific for each plant species, presenting a highly conserved TCP domain. In plants, they play a key role in the development and metabolism processes. In this study MaTCP genes have been updated, so, 46 MaTCP, 12 MbTCP and 25 MiTCP genes were identified in Musa acuminata, Musa balbisiana and Musa itinerans genomes, respectively. Chromosome location, gene structure, sequence alignment, gene classification and conserved domains of MaTCP, MbTCP and MiTCP proteins were predicted and analyzed. Our phylogenetic analysis classified MaTCP, MbTCP and MiTCP genes into two classes, which was further supported by the conserved motif structure and exon-intron organization. Inquiry in the promoter region suggested the possible roles of TCP banana genes in biological and physiological processes. RNA-seq analysis indicated MiTCP3-MiTCP5, MiTCP9, MiTCP20, MiTCP21, MiTCP33, MiTCP41 and MITCP44 took part during wild banana responsiveness at both normal and low temperatures growing. Gene evolution indicated a high conservation level within monocots TCP genes through orthologous identification between banana, rice, maize and Arabidopsis. Moreover, similar functionality and a high level of conservation were also seen through homology relationship analysis between MiTCP and MbTCP genes with MaTCP genes. The present study provides valuable information about characterization, evolution, diversity and functionality of MaTCP, MbTCP and MiTCP genes and will contribute to better understanding of the biological and physiological roles of TCP-TF in plants.