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Glycosaminoglycan model glass substrates and cancer cell interactions

Abstract:

We have analyzed the the static adhesion of three cancer cell lines of varying metastatic potential to surfaces functionalized with glycosaminoglycans (GAGs) in relation to GAG charge per dimer and GAG chain length. Four different GAGs (heparan sulfate (HS), chondroitin sulfate A (CSA), chondroitin sulfate C (CSC) and keratan sulfate (KS)) were deposited on glass surfaces that had been previously coated with 3-aminopropyltriethoxy-silane (APTES), and the substrates were used in static adhesion experiments. GAGs were strongly bonded to the silanized glass cover slips. The best substrate for adhesion was HS while KS was the least preferred substrate. The observed adhesion of whole cancer cells showed that a functional dependence may exist with the number of charges per dimer of the polyelectrolyte to which they are attaching and that this dependence takes the form of a linear function that increases with the number of charges per dimer.