Gravitropic response in radiata pine seedlings. Searching molecular keys

Abstract:

Coniferous trees develop compression wood in response to gravitropic stimuli. In nature this response can be generated by growth in slope, exposure to snow or high winds [1]. However, genes and molecular mechanism involved in this phenomenon are still unknown. We studied gene expression in response to gravitropic stimulation induced by 45 inclination in Pinus radiata D. Don one year old seedlings. To characterize the gravitropic response, whole seedlings were inclined and transversal cuts were performed in order to identify morphological wood characteristics. Xylem cells were visualized by optical microscopy in a time course experiment (fig. 1). On the other hand, a transcriptomic approach was assayed generating libraries based on the Suppressive Subtractive Hybridization (SSH) strategy [2]. This technique allows the isolation of genes differentially

Año de publicación:

2011

Keywords:

Fuente:

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