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HPLC/DAD analysis of phenolic compounds from lavender and its application to quality control

Abstract:

A reversed phase HPLC procedure is proposed for the determination of eight phenolic compounds (2-O-glucosilcoumaric acid, o-coumaric acid, rosmarinic acid, apigenin-7-O-glucoside, coumarin, herniarin, luteolin, and apigenin) in lavender. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS 2 (5 μm particle size: 25.0 x 0.46 cm) column. From the several extractive solvents assayed, only ethanol was able to extract all the mentioned compounds. Best resolution was obtained using a gradient of water-formic acid (19:1) and acetonitrile. Ten samples were subjected to quantification, all of them showing a common composition pattern.

Año de publicación:

2000

Keywords:

    Fuente:

    googlegoogle
    scopusscopus

    Tipo de documento:

    Article

    Estado:

    Acceso restringido

    Áreas de conocimiento:

    • Química analítica
    • Bioquímica

    Áreas temáticas:

    • Química analítica
    • Química orgánica
    • Tecnología de las bebidas

    Contribuidores:

    Amaral J.Amaral J.
    Moreira M.Moreira M.
    Filipe Miguel AreiasFilipe Miguel Areias
    Andrade P.B.Andrade P.B.
    Valentao P.Valentao P.
    Seabra R.M.Seabra R.M.