Heterologous expression of triterpene biosynthetic genes in yeast and Subsequent Metabolite Identification Through GC-MS

Abstract:

Heterologous expression of plant metabolic enzymes in microorganisms is extensively used for identifying genes involved in their pathways and producing useful compounds. Here, we describe a plasmid-based yeast expression system that easily allows the expression of different triterpene biosynthetic genes in wild-type yeast, providing a useful platform for identifying their functions and facilitating combinatorial biosynthesis of triterpenoids.

Año de publicación:

2014

Keywords:

• Cytochrome P450 reductase (CPR)
• Triterpenoid
• 2
• Cytochrome P450 monooxygenases (CYPs)
• 3-Oxidosqualene
• Yeast
• Combinatorial biosynthesis
• Triterpenol
• Oxidosqualene cyclases (OSCs)

Fuente:

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scopus