Human Bence Jones protein toxicity in rat proximal tubule epithelium in vivo

Abstract:

Human Bence Jones protein toxicity in rat proximal tubule epithelium in vivo. To investigate the direct toxicity of human Bence Jones protein (BJP), individual nephrons of male Sprague-Dawley rats were perfused in vivo at 20 nl/min with an artificial tubule fluid (ATF) that contained no protein, a human kappa BJP (5 g/dl), or bovine serum albumin (5 g/dl), and proximal convoluted tubule function and morphology were examined. Perfusion with BJP perfusate for ≤5 minutes produced no changes (P = NS) in absorption of water, Jv, (1.09 ± 0.20 vs. 1.50 ± 0.25 nl/min/mm), chloride, Jcl, (95 ± 47 vs. 123 ± 41 pEq/min/mm), and glucose, JG, (39 ± 3 vs. 40 ± 5 pmol/min/mm) compared to perfusions with only ATF. However, perfusion for at least 20 minutes with the same BJP perfusate produced decreased (P < 0.025) in Jv (0.58 ± 0.12 vs. 1.15 ± 0.14 nl/min/mm) and JG (27 ± 3 vs. 38 ± 3 pmol/min/mm) compared to …

Año de publicación:

1987

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Fuente:

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