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Hypoglycemic activity of jungia rugosa less on induced diabetic mice (mus musculus)

Abstract:

Jungia rugosa Less is a plant species that belongs to the Asteraceae family and it is distributed in the Andean region. This study has been focused on evaluate the hypoglycemic activity of Jungia rugosa leaves from Ecuador and determine its flavonoids content. The flavonic fraction (aglycones) was obtained from J. rugosa by reflux using ethanol 70%, methanol and ethyl acetate. The determination of flavonoids content was carry out through HPLC. The hypoglycemic activity of the flavonoid fraction was performed on induced severe and mild diabetic mice, using extracts at doses of 25, 100 and 400 mg/kg and standard controls (acarbose 20 mg/kg, insulin glargine 0.5 mmol/kg). Glucose levels were reduced in both severe diabetic and mild diabetic mice treated with J. rugosa extracts. In mild diabetes, the hypoglycemic activity of J. rugosa was statistically similar to acarbose. Flavonic fraction was not statistically similar to insulin treatment for severe diabetic mice. This activity is related to the presence of luteoline and apigenin, flavonoid aglycones found through HPLC analysis. Based on the results of this research, it can be concluded that J. rugosa is an interesting source of natural hypoglycemic compounds. Further detailed phytochemical, toxicity and clinical studies are needed.