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Identification of peptide mimics of the epitope recognized by CB-EGF1, a monoclonal antibody EGF specific

Abstract:

As part of an integral structure and function study, we characterized the natural epitope on the EGF recognized by the CB-EGF1 monoclonal antibody, by using peptides displayed on the coat protein of filamentous bacteriophages. Data analyzed demonstrated that the CB-EGF1 monoclonal antibody recognizes a conformational epitope, comprising aminoacids S9, H10, Y13, K28, E40 and R41. The identified peptide sequences are mimicking the region on the EGF interacting with the b-chain of the EGF receptor (EGFR). Synthetic peptides F22-54 and F22-55 compete with the EGF for binding to the CB-EGF1 antibody, as demonstrated by ELISA, but lacking biological activity in the EGF-EGFR system.