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Identification of rapd marker linked to resistance of Musa to Fusarium oxysporum

Abstract:

Fusarium wilt of banana is widely regarded as one of the most destructive plant diseases. It is caused by the soil-inhabiting fungus Fusarium oxysporum f. sp. c-bense. There is no chemical control available and the only way to control it is to utilize cultivars that are resistant/tolerant to the pathogen. The development of molecular techniques allows the determination of markers linked to certain conditions of economic importance and constitutes a strategy for fast, reliable and reproducible selection, accelerating the improvement through knowledge, in early states, of the reaction to a given characteristic and its interaction with the genotype. In this study, a RAPD marker linked to resistance of Musa to F. oxysporum is reported. Eight susceptible and ten resistant/tolerant entrances evaluated in the field were used. The RAPD patterns obtained with the primer OPK-03 detected a major fragment of 485bp, only found in the susceptible entrances and absent in the resistant/tolerant entrances. The study of genetic relations between the genotypes susceptible and resistant/tolerant to F. oxysporum, using RAPD primers, defined the existence of an associated polymorphic pattern to the resistance to the disease.