61. Prevention of liver cirrhosis development in rats with recombinant SV40 vectors expressing IGF-I

Abstract:

One of the landmarks of advanced liver cirrhosis is the decrease of serum IGF-I levels probably due to its diminished synthesis from liver parenchyma. Patients with liver cirrhosis have a number of systemic derangements related to the reduced levels of circulating IGF-I. The administration of recombinant IGF-I in physiological doses reverts these symptoms and partially, the cirrhosis state. Recombinant viruses expressing a therapeutic protein are being used as an alternative for recombinant protein administration. We decided to study the efficiency of an IGF-I expressing recombinant vector based on Simian Virus 40 virus (SV40) to prevent liver cirrhosis in a rat model. Recombinant SV40 vectors have several advantages for this purpose. They efficiently infect hepatocytes, they have a long-term expression and they are non-immunogenic. We have produced recombinant viruses expressing firefly luciferase (rSVLuc …

Año de publicación:

2004

Keywords:

Fuente:

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