648. In-Vivo Adenovirus Mediated RNA Interference of Multidrug Resistance Protein-2 (MRP-2/ABCC2/cMOAT)

Abstract:

RNA interference (RNAi) is a natural process, originally described in Caenorhabditis elegans and plants, initiated by the cleavage of long double stranded RNA (dsRNAs) by the ribonuclease III Dicer generating short interfering RNAs (siRNAs). siRNAs induce selective degradation of mRNAs that are homologous to their sequence. In mammalian cells, RNAi interference can be achieved by transfection of chemically synthesized siRNAs or short-hairpin RNA (shRNAs) precursor expressing plasmids. In-vivo gene silencing of endogenous and exogenous genes has been proven by hydrodynamics injection of both shRNAs and synthetic siRNAs, or by using shRNA expressing viral vectors such as lentivirus or adenoviruses. We have constructed first generation recombinant adenoviruses encoding different shRNAs against murine multidrug resistance protein 2 (MRP2/ABCC2/cMOAT)(AdshMRPs). Mutations in MRP2 …

Año de publicación:

2004

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Fuente:

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