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71 Inactivation of phospholipase C from rat basophilic leukemia cells by diisopropylfluorophosphate

Abstract:

asthma. We have recently described a series of potent, selective. 5-llpoxygenase lnhlbltors one of which, zlleuton (A-640771, Is under cllnlcal evaluation In asthma. In thls study, we sought to determine the ablllty of zileuton and other B-llpoxygenase Inhibitors to prevent lonophoreinduced leukotrlene release from human and guinea plg chopped lung. Zlleuton. A-631 62, and phenldone were more potent at Inhibiting leukotrlene release from human chopped lung than gulnea plg chopped lung. One possible reason for the weaker Wtencles of compounds In gulnea pig lung leukotrlene synthesis was that the matrix of the gulnea pig lung somehow Ilmlted access of the Inhibitors to the target enzyme. However, In prellmlnary studies. the potency for lnhlbltlon of leukotrlene release from gulnea pig lung monodlsperse cells by these compounds were similar to their potency In gulnea plg chopped lung. Interestingly, these …