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Implementation and usefulness of pyrosequencing in viral populations of patients with HIV-1 in Ecuador

Abstract:

Objective: implement the pyrosequencing system Roche/454 for the gene pol of HIV-1. Methods: based on plasma samples from 33 Ecuadorian HIV-1 positive patients with antiretroviral therapy failure, pyrosequencing was conducted and the results were analyzed with the DeepCheck software. Results: results were obtained for 27 of the 33 samples analyzed. Twenty-five mutations were found to be associated to resistance in viral populations. The most common were F77L, M184V/I, K103N/S, M46L/I, I50V/L, Q58E, V82T/F/A/S/L and L90M. High resistance was observed to non-nucleoside reverse transcriptase inhibitors and to protease inhibitors (43.5 and 44.4%, respectively, in minority populations), as well as to protease inhibitors in majority populations (26.4%). 2, 2 and 1 samples were detected with full resistance to each drug type (nucleoside reverse transcriptase inhibitors, non-nucleoside reverse transcriptase inhibitors and protease inhibitors, respectively) in majority populations, and 3, 4 and 6 in minority populations, as well as 2 viruses with resistance to the three drug groups in minority populations. Most samples belonged to subtype B (77.8%) in each of the study regions. Conclusions: the study allowed implementation of the pyrosequencing system, which made it possible to analyze the first data on resistance to antiretrovirals in Ecuador.