Improving the expression of mini-proinsulin in Pichia pastoris

Abstract:

Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achieved by increasing the cultivation pH from 5.1 to 6.3, by decreasing the temperature from 28 to 22°C, and by periodical addition of ammonium sulfate and EDTA to the culture broth. Using this procedure, mini-proinsulin reached nearly 0.3 g l -1 in the culture supernatant after 160 h of growth.

Año de publicación:

2004

Keywords:

• AOX promoter
• Recombinant human insulin
• PICHIA PASTORIS
• fed-batch culture

Fuente:

scopus

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