Regresar

In vitro susceptibility to fluconazole of Candida spp. isolates comparing three different methods

Abstract:

Introduction. Opportunistic fungal infections are an increasingly significant causes of morbidity and mortality particularly those related to Candida spp. As a consequence, there is an increase in the use of systemic antifungal agents as fluconazole responsible for the emergence of a new opportunistic fungi and resistant species. Purpose, materials and methods. In this study, two antifungal susceptibility testing systems, the Agar disk diffusion method and the Well diffusion test, were compared with the M27A-NCCLS reference broth microdilution method, using 36 isolates of Candida sp. (C. albicans, 15;C. tropicalis, 12;C. parapsilosis 5;C. glabrata 2;C. krusei, 1;C. lipolytica, 1). The antifungal drug tested was fluconazole. Results. The well diffusion test showed a specificity of 100% for resistant strains to fluconazole. While the sensitivity represented only 84.85%. The positive pbkp_redictive value for resistant strains was 100%. The disk diffusion test compared to the NCCLS had a sensitivity of 90.90% but a specificity of 66.67% for resistant strains. The positive pbkp_redictive value was 96.77%. Conclusions. We present a contribution to the standardization of in vitro susceptibility of yeast using the well diffusion method, and recommend it as a simple, inexpensive and reliable method.