Influence of solvents and methods of extraction in the determination of the antioxidant activity of the species" Disterigma alaternoides (Kunth) Nied." Native plant of ecuador

Abstract:

This research was developed to verify the influence of solvents and extraction methods in the antioxidant activity of the Disterigma alaternoides (Kunth) Nied species, the plant was subjected to a drying process and subsequently crushing to obtain the ethanol extract. Then, 50 grams of the fruit were subjected to different extraction processes using maceration, decoction and percolation methods, the solvents were (methanol-HCl 1%. 6: 1)(ethanol-Citric acid 0.03% P/V in water). The total phenol activity was determined by the colorimetric method of Folin-Ciocalteu and antioxidant activity in vitro by the method of 1-picrilhydrazile 2, 2 diphenyl radical (DPPH) in a concentration of 250 ug/ml of each extract. With the results of the concentration of total phenols was determined the antioxidant activity, it was of 97.73%, even greater than vitamin C 93.14%, that was used as reference standard. Finally, the obtained data were …

Año de publicación:

2019

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Fuente:

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