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Isolation and characterisation of a new Venezuelan strain of cassava common mosaic virus

Abstract:

A new strain of cassava common mosaic virus, designated CsCMV‐ve, was isolated from cassava (Manihot esculenta Crantz) plants growing in Venezuela. The 520 nm long semi‐flexuous virus particles sedimented in sucrose gradients as one component and contained a single coat protein molecule of c. 28 kDa, and a ssRNA of c. 2.1 times 106. A single dsRNA species of c. 4.2 times 106kDA was isolated from virus‐infected cassava leaves. In double antibody sandwich enzyme‐linked immunosorbent assay, CsCMV‐ve reacted strongly with antisera to CsCMV and potato virus X, but not with those to other typical members of the potexvirus group. Ultrastructural studies of CsCMV‐ve infected cells showed the presence of large bundles of inclusion bodies in the cytoplasm, a proliferation of the rough endoplasmic reticulum, and large crystals in the peroxisomes. CsCMV‐ve in crude buffered sap or in purified preparations was not mechanically transmitted to known hosts of the Chaya and Brazilian strains of CsCMV. Isolated virus RNA also failed to infect a range of test‐plant species. This is the first report of the presence of CsCMV in Venezuela. Copyright © 1995, Wiley Blackwell. All rights reserved