Isolation and characterization of eight microsatellite loci from Phaedranassa tunguraguae (Amaryllidaceae)

Abstract:

Phaedranassa tunguraguae is an endangered species endemic to Ecuador. Eight highly polymorphic microsatellite loci were isolated from an enriched genomic library for this species. Levels of polymorphism were evaluated using a total of 31 individuals from a single natural population. An average of 14.1 alleles per locus was detected, and observed heterozygosity ranged from 0.387 to 0.903. All but one locus depart significantly from Hardy-Weinberg equilibrium. These loci are the first microsatellite primers isolated for Amaryllidaceae and will be utilized to investigate patterns of genetic variation of P. tunguraguae, which will contribute data relevant to the conservation of the species. © 2005 Blackwell Publishing Ltd.

Año de publicación:

2005

Keywords:

• SSR
• Conservation
• Population genetics
• Microsatellite
• Phaedranassa
• Amaryllidaceae

Fuente:

scopus

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