Regresar

Isolation of lytic bacteriophages for Nanobiocontrol of pathogenic and antibiotic resistant Salmonella present in poultry in Ecuador

Abstract:

The genus Salmonella, as many others bacteria, has been frequently reported as resistant to commonly usedantibiotics in poultry, therefore, the current spread of antibiotic resistance genes in bacteria, raises doubts on theeffectiveness of antibiotics in the future. One possible alternative to antibiotics is the use of bacteriophages asantimicrobial agents to control antibiotic resistant pathogenic bacteria. The main goal of this project was to isolateand use bacteriophages as a new, safe, and effective biocontrol method for treating infectious diseases caused bySalmonella entero-pathogena. Four lytic bacteriophages cocktails (PSEA-2, SSEA, PSIA-2, SSIA) were isolatedfrom wastewater of poultry processing industries, to control Salmonella enterica subsp entérica serovar Enteritidis(SE), and Salmonella entírica subsp enterica serovar Infantis (SI), under laboratory conditions. We found thecocktails PSEA-2 and PSIA-2 specific for SE and SI while cocktails SSEA and SSIA also caused lysis inPseudomona. The observations in the Transmission Electron Microscope (TEM) revealed the presence of tailedphages of the Siphovididae and Myoviridae families; and a polyhedral phage. We have isolated specific phages andtested in vitro their effectiveness at controlling Salmonella. Further studies are needed in order to assess the phageeffectiveness in vivo.