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Isolation of microsatellite loci for the predatory fish Hoplias microlepis (Characiformes: Erythrinidae) from a highly impacted river system in western Ecuador

Abstract:

We developed 14 polymorphic, dinucleotide microsatellite loci for Hoplias microlepis, a large predatory fish from a highly impacted river in western Ecuador. These were isolated using a next-generation pyrosequencing approach and tested in a sample of 32 fish. None of the loci were in linkage disequilibrium and all appeared to be in Hardy-Weinberg. Nine of the loci exhibited greater variability, having 3-8 alleles (average = 5. 22), observed heterozygosities between 0. 22 and 0. 81 (average = 0. 58), and expected heterozygosities between 0. 20 and 0. 83 (average = 0. 56). The other five loci had 2 alleles and heterozygosity values generally close to 0. This is the first study dedicated to isolating microsatellites for a freshwater fish from the highly impacted aquatic ecosystems of western Ecuador. © 2012 Springer Science+Business Media Dordrecht.