Isolation, culture, cryopreservation, and identification of bovine, murine, and human spermatogonial stem cells

Abstract:

Spermatogonial stem cells (SSCs) are the germ cells at the basis of spermatogenesis in adult mammals. SSCs offer many biotechnological possibilities and are fundamental cells in the study of spermatogenesis (Aponte, World J Stem Cells 7:669–680, 2015). This chapter describes detailed procedures for SSC isolation, culture, cryopreservation, and characterization in bovine, murine, and human models.

Año de publicación:

2020

Keywords:

• Functional test
• cell culture
• Spermatogenesis
• Spermatogonial stem cells
• characterization
• Isolation
• Cryopreservation
• Testis

Fuente:

google

scopus