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Many broadly-shared mycobionts characterize mycorrhizal interactions of two coexisting epiphytic orchids in a high elevation tropical forest

Abstract:

Interspecific interactions play an important role in community assembly. A basic ecological question is whether interactions are specialized (one to one) or generalized (many to many). Specialization of interactions should ideally be assessed across several populations because species could be specialists at a particular site but generalists when several sites are considered. Mycorrhizal interactions are fundamental for orchid life and distribution, but their level of specialization is still under debate. To understand the extent to which epiphytic orchids are specialists in their mycorrhizal interactions, we studied the richness and phylogenetic structure of mycobionts across different sites, and the similarity in the mycobiont composition between coexisting orchid species. We sequenced the nrDNA ITS2 region and explored the mycobiont communities associated with two epiphytic orchids, Epidendrum marsupiale and Cyrtochilum pardinum, at two elevations within two sites in Ecuador. We found 108 OTUs belonging to Serendipitaceae (66), Ceratobasidiaceae (22), Atractiellales (11) and Tulasnellaceae (9). Orchids at the highest elevations hosted the highest OTU richness. The two orchid species shared a high percentage of mycobionts between all sites. No phylogenetic structure within orchid mycorrhizal communities was found at any sites or elevations. Our results indicate that the studied orchids are generalists and share a broad group of mycobionts (16 OTUs) with no apparent niche segregation within or between sites.