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Metabolism of the dibenzothiophene by Pseudomonas stutzeri PLC 16

Abstract:

In this work, the degradation of dibenzothiophene (DBT) by Pseudomonas sutzeri PLC 16 was evaluated by the study of the bacterial growth kinetic in the presence of this hydrocarbon as only carbon source, and the analysis of its metabolites of degradation by UV spectrophotometry. The kinetic growth of Pseudomonas sutzeri PLC 16 was determined by the quantification of the bacteria number through the ephifluorescence microscopy technique. The bacterial oxidation of the DBT was evaluated through the appearance of the 3-hydroxy-2-formyl benzothiophene and trans-4-[2-(3-hydroxy)-thionaphthenyle]-2- oxo-3 butenoic acid, which are the main metabolites in the degradation of this compound. As result, a rate growth of the culture of 0.828 h-1 with a time of duplication of 0.837 hours were observed. The dibenzothiophene oxidation was verified observing the appearance of the metabolite accumulation maximum peaks at 48 and 96 hours, a period that agreed with the logarithmic growth phase of the culture, condition that evidence the oxidation of the dibenzothiophene by P. stutzeri PLC16 and the potential of this strains for future biotechnological applications. © 2011 2002 Revista Técnica. Facultad de Ingeniería. Universidad del Zulia.