Metagenomics tools and SEM applied to identify a pool of corrosive sulfate-reducing bacteria (SRB) from naphtha transporting pipelines of Venezuelan Oil Industries

Abstract:

Different groups of bacteria such as SRB, aerobic iron-oxidizing, fungi, and others, have been reported as promoter of localized damage in metal structures. It has been estimated that 40% of the internal damage in transporting pipeline distribution is attributed to microbially-induced corrosion (MIC). The SRB group reduces sulfate and thiosulfate to sulfide (highly corrosive) in anaerobic environment and is frequently detected in storage and transport facilities in oil industries. Researches focused on identifying microbial species involved in corrosion are essentials for developing new approaches for detection, monitoring and control of MIC. Molecular biology techniques like DNA cloning and sequencing and Denaturing Gradient Gel Electrophoresis (DGGE), and others, have become powerful tools for the studies of biodiversity and/or functionality of microorganism’s communities, because allowed to identify both cultivable and non-cultivable microorganisms present in the environment samples. The aims of this work were applied metagenomics tools to molecularly identify a pool of SRB from naphtha transporting pipelines and evaluate their corrosive potential on coupon steel metal surface. Isolation of total DNA from positive sulfate reducer’s group culture medium (APIL and APIA), PCR amplification of 16S rRNA gene, DNA cloning and sequencing was performed for molecular identification. The results obtained were confirmed by DGGE technique. Scanning electron microscopic (SEM) was using to evaluate the corrosive potential of pool SRB. Three SRB species were found: Desulfovibrio sp., Soehngenia sp. and, Soehngenia saccharolytica. The …

Año de publicación:

2012

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