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Microsatellite markers developed for the Caribbean palm Pseudophoenix sargentii: Two PCR-based methods

Abstract:

Pseudophoenix sargentii is critically endangered in the USA. We have developed ten polymorphic microsatellite loci for this palm. A study based on populations from the Bahamas and Florida identified 3-17 alleles per locus. Values for the fixation index were positive, and eight loci deviated significantly from Hardy-Weinberg Equilibrium. Two different PCR-based methods were utilized. The first method yielded specific primerpairs for three of the loci, and it was based on amplification, cloning and sequencing of products obtained through 5′ anchored PCR. Specific primers for the remaining seven loci were obtained by refining microsatellite regions discovered by 5′ anchored PCR using a new protocol based on inverse PCR. © Springer Science+Business Media B.V. 2010.