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Molecular characterization and cryopreservation of fungi and bacteria associated to the rhizosphere of heavy metal accumulation vegetable species in soils contaminated by mining

Abstract:

Microorganisms are a clear example of the enormous biodiversity contained in the soil. It proceeded to the in vitro isolation, molecular characterization and identification of microorganism's rhizosphere soil associated with Miconia zamorensis and Erato polymnoides, regarded as accumulators of heavy metals in the mining area of Chinapintza - Zamora Chinchipe. The strains were identified by sequencing the 16S ribosomal RNA gene in bacteria, and it was identified the presence of genera such as Bacillus, Serratia and Lysinibacillus. In fungi was used the sequence of ITS ribosomal RNA gene and it was identified the genres Aspergillus, Talaromyces, Penicillium, Trichoderma, Nodulisporium, Fusarium, Rhizopus, Mucor, Hypochnicium and Umbelopsis. Such genres form part of the soil and are considered as tolerant to ecosystems with heavy metals contamination. Our results will be the starting point of information on the potentiality of these microorganisms to develop bioremediation strategies in the study area. Due to the high demand to elaborate collections of microorganisms for bioremediation purposes, it was proposed the cryopreservation of bacterial and fungal pure cultures. It was obtained an effective viability in the reactivation of bacterial cultures, while in fungi most cultures were reactivated successfully.