Molecular diagnosis of Canine Parvovirus in geriatric dogs

Abstract:

The objective of this study was to determine the presence of Canine Parvovirus in geriatric dogs that attended consultation with gastrointestinal symptoms compatible with canine parvovirus in the period from November to December 2021 in a Veterinary Clinic in the South of Quito, Ecuador by molecular diagnosis (PCR), stool samples were taken from the anus with a swab and homogenized (10 % w/v) in RNA Latter solution. Suspended faeces were separated by centrifugation and 200 mu L aliquots of supernatants were used to extract DNA. For the design of primers and probes from GenBank, VP2 nucleotide sequences were retrieved from some CPV-2 strains, which were aligned using Primer3 software. The numbers and strains used for the alignment were: CPV2: CPV-b, M38245 and CPV-Northern, M19296; CPV2a: CPV-15, M24003 and CPV-31, M24000; CPV-2b: CPV-39, M74849 and CPV-133, M74852 …

Año de publicación:

2022

Keywords:

Fuente:

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