Monoclonal Antibodies Binding to Malarial Merozoite Surface Protein 1 Protect in vivo Against Plasmodium yoelii Infection

Abstract:

The malarial merozoite invades the erythrocyte and develops within the host cell. One of the most important and well studied parasite surface proteins at this stage of the life cycle is merozoite surface protein-1 (MSP-1). Plasmodium falciparum MSP-1 undergoes proteolytic processing into several fragments, of which only the carboxyl-terminal 19 kDa fragment (MSP-119) remains on the merozoite surface during invasion of a new erythrocyte. To study the importance of antibodies to the 19 kDa fragment of Plasmodium yoelii MSP-1 in providing protective immunity against infection, MSP-1-specific monoclonal antibodies were produced and characterised. Six antibodies were studied in detail. When naive mice were passively immunised with these antibodies and then challenged with P. yoelii YM; which is a virulent and lethal parasite line, four of the antibodies showed a protective effect, reducing the level of …

Año de publicación:

1997

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