Morphological quality of in vivo oocytes recovered in diferent follicular waves from FSH-treated cows

Abstract:

The objetives of the present work were determine 1) the effect of FSH treatment and 2) the estrous cycle period during the follicu-lar wave (FW) (metaestrus = first follicular wave or diestrus = second follicular wave) development on the number of visualized and aspirated follicles and number of cumulus-oocyte complexes (COC) recovered (ovum pick-up=OPU) and number of viable COC. Angus adult cows (n=28) were allocated to four treatment Groups. G1: OPU on day 4 (day 0=ovulation), G2: FSH on days 0, 1 and 2 (66.6 mg/day, Folltropin®) and OPU on day 4, G3: OPU on day 13, G4: FSH on days 9, 10 and 11 and OPU on day 13. In the Groups 3 and 4, dominant follicle aspiration was performed on Day 8 to synchronize the second follicular wave. A total of 30 OPU sessions per Group was performed. The mean (±SEM) numbers of aspirated follicles were 11.3 (±0.6) and 2.4 (±0.1) to FSH-treated cows and non-treated cows respectively. The mean number of follicles aspirated was not different (P>0.05) between follicular waves. The mean numbers of recovered and viable COC were greater (P<0.05) in FSH-treated cows than those in non-treated cows regardless of FW. Oocytes recovery rate (recovered COC/aspirated follicles x 100) in FSH-treated cows (35.5%) was lower (P<0.001) than that in non-treated cows (70.9%) regardless the FW. We can conclude that 1) FSH treatment increase the number of COC and viable COC in vivo recovered by means of OPU and 2) a great variability in the response of these reproductive parameters was observed, according to the period of the estrous cycle (metaestrus or diestrus) in which the FW is developed, due to the presence or functionality of corpus luteum and the physiological differences of the follicular populations.

Año de publicación:

2020

Keywords:

• Follicular wave
• Oocyte
• Fsh
• bovine
• Ovum pick-up

Fuente:

scopus