Mutation induction by Gamma-rays and carbon ion beam irradiation in banana (Musa spp.): a study with an emphasis on the response to Black sigatoka disease

Abstract:

[en] Gamma-rays and carbon ion beam irradiation methods were applied to study critical doses, genetic variability and the response to Black sigatoka disease.'Cavendish Enano','Williams','Orito'and'FHIA-01'cultivars of banana were studied. Both gamma-rays and carbon ion beam irradiation methods had different biological effects when banana explants were exposed to them. In both methods, increased dose caused increased mortality.'FHIA-01'tolerated high doses of gamma-rays but was susceptible to high doses of carbon ion beam irradiation. The results suggest that the response in'FHIA-01'can be explored using other dose intervals between 150 and 300 Gy. Weight and height were also reduced drastically when high doses of gamma-rays and carbon ion beams were applied. The LD50 of cultivars' FHIA-01'and'Orito'revealed high sensitivity to both gamma-rays and carbon ion beams. DNA deletion in'FHIA-01'occurred by using gamma-rays at doses of 200 and 300 Gy, suggesting that'FHIA-01'is definitely a promising cultivar with a high sensitivity response to gamma-ray exposure, and that there is a high chance of improving its fruit quality by mutation induction. Sigmoid drooping leaf, a putative mutation of'FHIA-01', was generated. This mutation is heritable as mother plant and sucker showed the same characteristics. Future research could be conducted on the relationship of leaf shape to fruit quality and production. Hexaploid cells were detected by flow cytometry (five plants in'Cavendish Enano'and one in'Williams'), signifying that chromosome duplication can be induced by carbon ion beams. Variation in the leaves such as being …

Año de publicación:

2010

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