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Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins

Abstract:

A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria. © 2009 American Chemical Society.

Año de publicación:

2009

Keywords:

  • M. Tuberculosis
  • ConA affinity, Lipoproteins
  • Mannosylated

Fuente:

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scopusscopus

Tipo de documento:

Article

Estado:

Acceso restringido

Áreas de conocimiento:

  • Bioquímica
  • Microbiología
  • Proteína

Áreas temáticas:

  • Microorganismos, hongos y algas

Contribuidores:

Parada C.Parada C.
Hernández G.M.Hernández G.M.
Bigi F.Bigi F.
Espitia-Pinzon C.I.Espitia-Pinzon C.I.
Xolalpa W.Xolalpa W.
Antonio Javier Vallecillo MazaAntonio Javier Vallecillo Maza
González-Zamorano M.González-Zamorano M.