Non-intrinsic contribution to the limiting partial molar volume of globular proteins in water: a study comparative between a new refractometric strategy and densitometric …

Abstract:

The partial molar volume of a globular protein is considered a property constituted for by two volumetric contributions, an intrinsic contribution (volume of impenetrable to the solvent) and a non-intrinsic one (volumetric contribution from repulsive and attractive interactions). The aim of the present study is to determine the non-intrinsic contribution to partial molar volume of BSA, HSA and OVA as globular protein model at infinite dilution in free salt water at 298.15 K using a refractometry strategy and the classical densitometry method. To this end, we used several models reported in the literature based in the linear polarization theory and empirical correlation to calculate the levels of refraction and the average molecular polarizability and intrinsic molar volume for each protein at low-range concentrations. We found that the intrinsic volumetric contribution values for each protein using the refractometry strategy were …

Año de publicación:

2015

Keywords:

Fuente:

google