Novel techniques for the screening of microbial compounds

Abstract:

In Ecuador around 15,277 cases of non-inflammatory disorders of sexual organs caused by Candida albicans are reported annually. The increase of clinical reports of candidiasis is mainly caused by the absence of materials required for the characterization of Candida spp. This research is focused on developing new methods for screening of microorganisms through the use of low-cost materials. We worked testing several laboratory materials such as microscope slides, microcapillary tubes, cover glasses, and Pasteur pipettes along with certain substances such as blood serum, N-Acetilglucosamina (GlcNAc) and yeast extract-peptone (YP) solution to stimulate the growth of the microorganisms. Different growth systems were arranged and filled with different growth media; consequently, these samples were inoculated with Candida albicans and incubated at 37 C. It was observed that cold silicone showed better fixation properties and a lower amount of air bubbles when it is compared to the hot silicone. It was found that agar showed different behaviors at different levels of concentration in addition to the blood serum used for growth medium. Image clarity was also analyzed in all samples. The growth of Candida albicans was successfully observed in media with blood serum and yeast extractpeptone-dextrose (YPD), which can be extracted from the blood sample of the patient without affecting the outcome of the analysis. These techniques can be used to recognize unknown microorganisms; hence, antibiotic and antifungal resistance could be prevented.

Año de publicación:

2020

Keywords:

Fuente:

google